For particle volume estimates, a variety of local size estimators has been developed, beginning with assumption-based methods like planimetry. While stereology is applicable to all tissue, it proves to be an important technique that allows the investigator to quantify cell population parameters in late-responding and non-proliferative populations. The purpose of this paper is to compare the stereology methods to estimate cell volume. The in vitro and in vivo descriptive and clonogenic techniques used regularly in radiation biology and in clinical therapy will not be replaced by stereology.

This presentation reviews in broad terms the evolution of the need to quantify population and kinetic techniques and how the need has been met. Several methods can be used to estimate the reference volume. Alternatively, for cell number estimates, Fractionator sampling can be used (as described above), which is insensitive to shrinkage 9, 34. applied these techniques to quantify the population changes in the vasculature of the retina. Stereology avoids the bias of the reference trap through obtaining an unbiased estimate of the reference volume before any other parameters are measured. 000, 000РІР‚вЂњ000, 2003) and earlier papers by Archambeau et al. We estimate the reference volume by point counting an optical section at the top of the disector that provides us with a reference area that is multiplied by the distance traveled in the Z direction for counting structures. 000, 000РІР‚вЂњ000, 2003) introduced to radiation biology the techniques of stereology required to quantify the dose response of irradiated brain populations. Knowledge of the reference volume is crucial to convert densities estimated with stereological test systems to total quantities SURS Systematic uniform random sampling. 160, 120РІР‚вЂњ123 (2003).Р”Р„ paper by Kubinova et al. Reference volume The space from which samples are taken and in which particular stereological measurements are performed, e.g. Stereology Techniques Have-or Should Have-a Role in Preclinical Radiation Therapy.

Stereology reference trap volume